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Responsibilities
- Tissue culture using both 2D and 3D (organoid) systems
- Extraction and analysis of DNA and mRNA, including PCR, quantitative PCR, molecular cloning
- Animal studies, including mouse colony management, breeding, and genotyping
- Assisting in animal surgeries and in vivo drug delivery
- Microscopy, immunohistochemistry, and immunofluorescence
- Flow cytometry, fluorescence-activated cell sorting (FACS)
- Lentiviral production and viral titering
- Data analysis and recording of results electronically and in a lab notebook
- Protocol and literature searches
- Evaluations of new assay methods or instrumentation
- Working closely with a post-doctoral fellow who will serve as a direct supervisor and mentor
- Co-operating with a team of experimental and computational scientists
- Ordering and stocking of lab supplies and reagents
- Preparation of reagents and media used in lab experiments
- Operation and maintenance of standard lab equipment
- Occasional pick up/delivery of patient samples
Highly motivated and talented research technicians are expected to become gradually independent and may eventually lead projects for which there is strong precedent in the laboratory.
Qualifications
Minimum qualifications include a bachelor’s degree in biology or a related discipline. Candidates should be familiar with standard wet lab techniques (e.g., tissue culture, PCR, western blot, DNA/RNA purification) and motivated to learn new protocols. The most critical factors for success in this position are a desire to learn and a commitment to the team. The research technician will be paired with a postdoctoral fellow or graduate student to learn and assist on projects in the laboratory. They will also meet with Dr. Freed-Pastor to assess progress and receive guidance. They will also help with everyday lab maintenance tasks such as ordering supplies and aliquoting reagents.
At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff. Cancer knows no boundaries, and when hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.
Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.